Boston Bioprocess Advantage

Our Capabilities

Discover what our fermentation platform capabilities can do for you.

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Bioreactors

*Recommended Max Working Volume
Physical Size Specifications Typical Max
Working Volume
Purpose
1.5 L
  • Up to 5 pumps for feeds
  • 3.5 m/s agitator tip speed
  • Up to 16 reactors in parallel per run
  • All bioreactors have advanced fermentation controls and programming on pH, DO, etc.

  • Off gas analyzer available

  • All bioreactors are glass vessels unless otherwise indicated
1 L*
  • High throughput strain screening
  • Rapid process onboarding
  • Media or parameters optimization
  • Accelerates early decision making
14 L
  • Up to 3 pumps for feeds
  • 4.7 m/s agitator tip speed
  • Up to 2 reactors in parallel per run
10 L*
  • Bridging 1.5 L discovery with larger-scale development
  • Supports early DSP validation and sample generation
20 L
  • Up to 3 pumps for feeds
  • 5.0 m/s agitator tip speed
  • Stainless steel vessel
16 L*
  • Entry point to pilot-scale development, validate scale-up strategies
  • Generate initial batches for performance characterization
500 L
  • Up to 4 pumps for feeds
  • 6.0 m/s agitator tip speed
  • Stainless steel vessel
400 L*

A robust pilot-scale fermenter ideal for process parameter optimization:

  • For producing larger sample volumes
  • Supports regulatory development activities or pre-GMP feasibility batches
1500 L
  • Up to 4 pumps for feeds
  • 5.2 m/s agitator tip speed
  • Stainless steel vessel
1200 L*

Supporting sample generation for regulartory submissions, and late-stage process validation

Centrifugation

*Assuming 20% Packed Cell Volume (PCV) but throughput highly dependent on feed characteristics
*Assuming 30% Packed Cell Volume (PCV) but throughput highly dependent on feed characteristics
Equipment Specifications Capacity Purpose
Floor/Bucket Centrifuge
  • Thermofisher Lynx 6000
  • Temperature set points available
  • Up to 17,000 g RCF
  • 6 x 1 L containers per loading

Batch-based solid-liquid cell separation in small volumes. Ideal for processing when flexibility is important

Disk Stack Centrifuge
  • GEA - HSD1
  • Automatic discharge mode and Continuous nozzle discharge mode
  • 1 L bowl size
  • 10,000 g max RCF
  • 5 - 8 L/min* feed rate, continuous mode
  • 1 L/min* feed rate, discharge mode
  • 5,000 - 10,000 RPM

Used for solids (cells) liquid separation from fermentation broth

Nozzle type disc stack: Enables continuous separation of solids (cells) from liquids. Constant ejection of solids without interrupting centrifugation

Automatic Discharge Mode: Allows for periodic solids (cells) removal. Useful when feed variability or solids loading requires controlled discharge events

Disk Stack Centrifuge2
  • Alfa Laval Clara 20
  • Automatic discharge mode
  • 2.2 L bowl size
  • 11,000 g max RCF
  • 2 L/min* feed rate, discharge mode
  • 5,000 - 9,500 RPM
Disk Stack Centrifuge3
  • Alfa Laval Clara 200
  • Automatic discharge mode
  • 5 L bowl size
  • 10,000 g max RCF
  • 15 L/min* feed rate, discharge mode
  • 5,000 - 7,500 RPM
Decanter Centrifuge
  • Alfa Laval SG2-100
  • 3,140 g max RCF
  • 5.5 L/min** feed rate
  • Optimized for high solids, high PCV slurries

  • Effective for handling dense sludge or unconventional feedstocks

Cell Lysis

Equipment Specifications Capacity Purpose

Bead Mill

  • WAB Dyno-Mill KDL
  • Bead type and size are process dependent
  • 1.4 L bead mill chamber volume
  • 1 - 50 L/hr processing range

General purpose bead mill for cell lysis, suitable for various applications

  • Can handle solid and liquid samples
WAB Dyno-Mill KD20 (Bead Mill)
  • WAB Dyno-Mill KD20
  • Bead type and size are process dependent
  • 16.5 L bead mill chamber volume
  • 70 500 L/hr processing range

For larger scale grinding and dispersion compared to the Dyno-Mill KDL

  • Can handle solid and liquid samples
GEA Panda 2000 (2-stage homogenizer)
  • GEA Panda 200
  • Bead type and size are process dependent
  • 9 - 20 L/hr, dependent on pressure

High-pressure homogenizer for cell lysis (reduces particle size, disrupts cells, creates uniform mixtures)

  • Appropriate for liquid samples
Chemical Lysis

Process dependent

Refer to the auxiliary equipment slide

Option to perform chemical cell lysis if needed (Heat sensitive protein, protection from denaturation, etc)

Dead End Filtration

*Varies depending on feed characteristics
Equipment Specifications Capacity Purpose
Depth Filtration
  • Pico Plate & Frame Filter 30 Plate
  • 0.1 - 15 um pore size (not absolute)
  • Up to 1.2 m2, total surface area
  • 2 - 10 L/min*, filtration rate

Serves as a pre-filtration or clarification step to remove large particulates, cell debris, or aggregates before finer filtration

Lenticular modules are highly scalable for larger batches or modular expansion

Depth Filtration2
  • Filtrox 16" Lenticular Filtration
  • 0.04 - 35 um pore size (not absolute)
  • Up to 10.8 m2 total surface area
  • 2 - 10 L/min*, filtration rate
Capsule Filtration

Sterlitech and any other brands

Varies depending on brand, chemistries and sizes but can accommodate all vendors’ specifications

A ready-to-use filtration solution combining housing and filter media in a compact format

  • Ideal for small-scale or single-use applications

 Cartridge Filtration

  • 2 x 30” Waco filter, up to 2 m2
  • 0.1-1.2 um pore sizes (absolute)
  • 2.0 m2 per housing for 30” cartridge
  • 2 - 10 L/min*, filtration rate

Provides robust filtration for medium to large process volumes

Compatible with multiple pore sizes for staged filtration. Suitable for critical clarification and polishing steps

Cartridge Filtration2
  • 2 x 20” Shelco filter, up to 1.34 m2
  • 0.1 - 1.2 um pore sizes (absolute)
  • 1.34 m2 per housing, filter area
  • 2 - 10 L/min*, filtration rate
Bag Filters
  • Cloth, plastics bag filters, pore size ranges from 1.0 um and above
  • 100 - 200 um max, polyester

Varies depending on brand, chemistries and sizes but can accommodate all vendors’ specifications

A cost-effective bulk filtration option

  • Commonly used for initial clarification of high-load process streams or non-critical filtration

Chromatography

Equipment Specifications Capacity Purpose
AKTA Avant
  • Cytiva - AKTA Avant 150 (Bench)
  • 3 wavelength monitor
  • Quaternary Pump for buffer scouting
  • Filter choice is highly process dependent

Up to 150 mL/min (9L/hr)

Bench scale, chromatography development for high purity applications and projects

AKTA Process
  • Cytiva - AKTA Process 10mm (Pilot)
  • 3 wavelength monitor
  • Filter choice is highly process dependent

Up to 10,000 mL/min (600 L/hr)

Pilot scale, chromatography development for high purity applications and projects

Tangential Flow Filtration

*SA = Surface area
Equipment Specifications Capacity Purpose
Ceramic Membrane
  • Shan Dong Bona Group
  • Membrane = Alumina, Zirconia

0.095 m2 filter SA

Pressure-driven separation, typically supporting volumes 10 - 20 L. Can be used for cell separation or further broth clarification

Ceramic Membrane2
  • GEA Model R with Atech ceramic membranes
  • Membrane = Alumina, Zirconia
1.0 m2 filter SA

Pressure-driven separation, typically supporting volumes more than 60 L. Can be used for cell separation or further broth clarification

Flat Sheet
  • Repligen TangenX (Bench)
  • Membrane = Any Repligen TangenX compatible membrane

0.5 m2 filter SA

Compact and flexible format for early-stage DSP process development, typically supporting volumes 1 - 20 L

Flat Sheet2
  • Repligen TangenX (Pilot)
  • Membrane = Any Repligen TangenX compatible membrane

5 m2 filter SA

Pilot-scale platform optimized for mid-scale DSP development more than 60 L

Spiral Wound

  • Sterlitech 1812 Membrane Test Skid
  • 1.8” Spiral wound TFF
  • Membrane = PES, PS, PVDF, etc
  • 0.3 m2 filter SA

Entry-level spiral TFF setup for process development at 10 - 20 L scale. Offers ease of handling and efficient surface area-to-footprint ratio

Spiral Wound2
  • GEA Model R
  • 2.5” Spiral wound TFF or 2 x 3.8” Spiral wound TFF columns
  • Membrane = PES, PS, PVDF, etc
  • 0.7 m² Filter SA (per 2.5” housing)

  • 7 m² Filter SA (per 3.8” housing)

Scalable TFF solution for larger DSP runs more than 60 L for sample generation. Ideal for both development and batch-ready membrane performance assessment

Drying

*Varies based on inlet temperature; **Varies based on solids loading and slurry characteristics
Equipment Specifications Capacity Purpose
Spray Dryer
  • Buchi 290 – Glass chamber
  • 120 C - 220 C inlet temp range
  • 2-fluid nozzle, top mounted (co-current)

Up to 2 L/day*

Lab scale spray dryer used for small batch production

Spray Dryer2
  • Pilotech YC-019 - Stainless Steel Chamber
  • 105 C – 300 C inlet temp range
  • 2-fluid nozzle, top mounted (co-current)
Up to 5L/hr*

Lab and pilot-scale spray dryer, popular for flexibility and cost-effectiveness

Spray Dryer3
  • Anhydro Spray Dryer - Stainless Steel Chamber
  • 105 C – 300 C inlet temp range
  • 2-fluid nozzle, bottom or top mount (co or counter current)

Up to 60 L/day*

Full-scale industrial spray dryer designed for large-volume, continuous production

Tray Drying
  • Thermo Scientific Lindberg
  • 30 mmHg vacuum capability
  • Temperature control

Up to 20 L scale**

Product that is heat-sensitive, oxidation-prone, or requires low-temperature drying for quality preservation

Tray Drying2
  • Temperature and humidity control

Up to 1500 L scale**

Drying stable products at a larger scale

Freeze Drying
  • Labconco Freezone
  • 3 x 11” x 9” trays per loading

Up to 20 L scale**

1 L – 2 L per loading**

Drying delicate/thermolabile materials. Precise control of temperature, vacuum, and drying profiles

Freeze Drying2
  • Harvest Right HRC-100
  • 7 x 29.5” x 11” trays per loading

Up to 500 L scale**

14 L – 21 L per loading**

Freeze drying at a larger scale

Auxiliary Equipment- Vessels & Unit OPs

Equipment Specifications Capacity Purpose
Tank – 200 L
  • CIP-able

200 L

Holding/processing tank

Feed Tank – 400 L
  • CIP-able
  • Steam//water jacket
  • Bottom mount mag mix agitator

400 L

Often used as feed tank to 500 L fermenter and can be used as holding/processing tank

Harvest Tank – 800 L
  • CIP-able
  • Steam//water jacket
  • Bottom mount mag mix agitator

800 L

Often used as harvest/dilution tank and can be used as holding/processing tank

Tank – 900 L
  • CIP-able
  • Steam//chilling jacket
  • Bottom mount mag mix agitator

900 L

Holding/processing tank
Tank – 2000 L Tank
  • CIP-able
  • Steam//chilling jacket
  • Top mount agitation

2000 L

Holding/processing tank
Vibratory Separator
  • 30” SWECO Vibratory Separator for hard pellet solids

Varies depending on feed characteristics

Cell separation process

Class I Div I Volatiles Room Equipment

Equipment Specifications Capacity Purpose
750 L Tank
  • Hastelloy reactor
  • Bottom mount mag mix agitator
  • Supports full vacuum
  • Steam//chilling jacket

750 L

Mainly used for solvent extraction processes with hazardous chemicals

Able to distill to or from any combination of these three tanks

900 L Tank
  • 316 Stainless steel
  • Bottom mount mag mix agitator
  • Supports full vacuum
  • Steam//chilling jacket

900 L

660 L Tank
  • 304 Stainless steel
  • Double-action (counterrotating) side-scrape agitated
  • Supports full vacuum
  • Steam//chilling jacket


660 L

Disc stack centrifuge
  • GEA SC-06
  • Explosion Proof
  • Max RCF = ~10,000 g
  • RPM = 5000 – 12150
  • 2-phase (solid-liquid) separation

Varies depending on feed characteristics

Rental unit - can support hazardous and flammable material

Analytical Methods

Equipment Equipment Type Possible Applications
Roche CEDEX Bio / Bio HT

UV-Vis Bioanalyzer, UPLC

In-Process Metabolites: glucose, glycerol, methanol, ethanol, acetate, ammonium, nitrate, total protein, calcium etc.

WATERS Acquity H-Class+

HPLC / UPLC

Product content and purity, metabolites, activity assays

Mettler Toledo HE53

Moisture Analyzer

Moisture Content / Total Dissolved Solids

BMG Spectrostar Nano

UV-Vis plate reader (microplates, cuvette)

Protein content (Bradford, BCA), ELISA, Enzyme activity

Thermofisher Xcell SureLock MiniGel Gel electrophoresis system Polyacrylamide

SDS PAGE/ Native PAGE

Thermofisher Xcell SureLock Blot Module Blotting system

Western Blot

Bio-Rad PCR and gel system PCR thermocycler, Gel electrophoresis Agarose

DNA multiplication and detection

Anton Paar DMA 35 Densitometer (hand-held)

Density measurements

Thermo Scientific Orion Turbidity meter Turbidity meter

Turbidity measurements

USS DVT6 Digital Viscometer Viscometer

Viscosity measurements

Thermofisher Genesys 30 and 50 UV-Vis spectrophotometer (cuvettes)

Photometry, Optical density, Activity assays

Additionally, we offer a variety of other analytical methods like: contamination checks, CFU counting, shelf-life studies, storage testing and many more. We have established relationships with external testing labs and will find external collaborators for our clients as needed.

External Testing Established External Partners
FDA certified Sample testing

Eurofins Scientific, Merieux NutriSciences

Mass spectrometry, Gas chromatography, FAME, flow cytometry, advanced microscopy, etc

University of Illinois UIUC, IBRL

Fermentation

1.5L & 15L Fermentation

1.5L & 15L Fermentation

20L Fermentation

20L Fermentation

500L Fermentation

500L Fermentation

Downstream Processing

TFF Systems

TFF Systems

DSC & Homogenization

DSC & Homogenization

Spray Drying

Spray Drying

Analytics

CEDEX

CEDEX

HPLC & Cell Viability

HPLC & Cell Viability

SDS PAGE & Western Blot

SDS PAGE & Western Blot